Immunocytochemistry analysis of Cas9 in HEK293 cells. Samples were stained with Cas9 monoclonal antibody (Product # MA5-47415) in red. Cells were transfected with a construct including the N-terminal 608 amino acids ofS. pyogenesCAS9 fused to GFP. Transfected cells express the green fluorescent fusion protein and bind the antibody in red, producing a yellow signal. Nuclear DNA in transfected and non-transfected cells is revealed with the blue DNA stain Hoechst.
Supplier Page from Thermo Fisher Scientific for Cas9 Antibody